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up to now, the exact binding site of MIF to CD74 just isn't crystal clear, but smaller molecules and mutations focusing on the tautomerase Energetic web site pocket have been shown to interfere Together with the binding of MIF to CD74 [44]. Interestingly, admirer et al. made just one cysteine mutant of MIF (MIFN110C) that covalently locks MIF into
MIF is required for your conversation among NLRP3 along with the intermediate filament protein vimentin, which happens to be important for NLRP3 activation. further more, we show that MIF interacts with NLRP3, indicating a role for MIF in inflammasome activation unbiased of its position like a cytokine. These information advance our idea of how MIF